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So I've have tried for days to try and figure out the equation for magnification of a camera verses the ocular lens on a microscope. The scope I am using is an AmScope SM-4TZZ-144A-18M3 3.5X to 180X magnification. The camera has a sensor size of 6.14x4.61mm and the calibration resolution is 4912 X 3684 (I have read this stuff matters but not how it works). The AmScope video on calibration is now almost 10 years old and doesn't explain the equation or anything. Say this is my set up oculars (which do not affect the camera) are 20x, my objective is at 4.5 (45 right?), and a Barlow of 2X. That is the max my scope will do and should be the 180X advertised. So what is my camera seeing at the setting since the ocular do not enter into the equation? This is all I am missing on getting the calibration done on my scope. I thought I had it done (and I might have had it done) until I realized the oculars are not involved with the camera. I'm asking all over because this is the new thing I have yet to understand. Please help me.